William M. Geisler, Robert J. Suchland, William L. H. Whittington, Walter Elizabeth. Stamm, Decimal Culture regarding Chlamydia trachomatis: omgchat Matchmaking from Addition-Creating Units Built in People so you’re able to Health-related Symptoms and you may Acute Tenderness from inside the Urogenital Condition, The newest Journal out-of Contagious Illness, Frequency 184, Material 10, , Profiles 1350–1354,
Abstract
The partnership off Chlamydia trachomatis introduction-creating devices into the quantitative community so you can scientific symptoms and you can pain for the urogenital situation is actually examined in 1179 clients planning to a sexually transmitted problems clinic. 450 ifu), count and you will character regarding cervical discharge, ?29 polymorphonuclear tissue (PMNL) for each and every higher-electricity profession (hpf) to the Gram spot (2050 vs. 320 ifu), and diagnoses off mucopurulent cervicitis (MPC; 2550 compared to. 300 ifu) and you can pelvic inflammatory problem (PID; 3000 against. 578 ifu). In the guys, higher addition-building product counts were regarding the urethral launch (85 vs. forty-two ifu), matter and you will character of discharge, and you will ?10 PMNL/hpf (95 versus. 50 ifu). These types of contacts carried on towards multivariate study. For this reason, chlamydial replication was of this MPC and you may PID in women, urethritis within the people, and you can inflammation in. Since the attacks with a high introduction counts could be the really transmissible, personality and you will treatments for people with this chlamydia-related syndromes is important in manage applications
Understanding the matchmaking away from Chlamydia trachomatis urogenital state so you can scientific symptoms and you can intense soreness leads to finest knowing the epidemiology and you may pathogenesis of those infections. Logical symptoms due to C. trachomatis almost certainly show the brand new combined ramifications of direct chlamydial replication and you can server inflammatory responses [ 1]. The connection between energetic chlamydial duplication and you may systematic signs is reviewed making use of the level of inclusion-creating gadgets built in cellphone structure society as a quantitative scale of the number of practical infectious primary government in the genital secretions. What number of inclusion-building systems from inside the people could be considered to be a good surrogate having infectivity or transmissibility [ 2]. Of the usage of a huge databases that desired having variations out-of potential confounding circumstances, we presented a combination-sectional investigation to evaluate the partnership between chlamydial introduction-forming systems from inside the quantitative cultures with clinical signs and you will degree of acute pain from inside the urogenital chlamydial illness
Strategies
Diligent inhabitants and you may data collectionThe studies society contained 671 people and you can 933 heterosexual males that has a culture-documented urogenital chlamydial disease during the Social Fitness, Seattle and you will Queen Condition Intimately Transmitted Disease Infirmary out of Could possibly get 1995 through December 1999. Investigation studies was indeed on clinic’s automatic databases, which includes sex-particular standardized medical record models. People that have coexisting gonococcal issues otherwise infection due to nonfusing C. trachomatis phenotypes were excluded as an easy way off addressing potential confounders one to ydial introduction-building equipment counts in order to health-related symptoms [ step 3, 4]. Clients that have recite infection with similar serovar inside thirty days or combined serovar infection was indeed excluded, because their inclusion manage violate independence about multivariate studies
Source and characterization ofC. trachomatis isolatesThe methods used for specimen collection, isolation of C. trachomatis identification and quantification of inclusions, and serotyping have been described in detail elsewhere [ 2, 5]. In brief, specimens were collected, were stored in chlamydia transport medium at 4°C, and were transported within 24 h to the laboratory. Each specimen was inoculated onto McCoy cells and overlaid with growth medium. Chlamydial inclusions were detected with a genus-specific antichlamydial lipopolysaccharide monoclonal antibody conjugated with fluorescein. The average inclusion count per 100-?L well was determined by counting 3 fields and multiplying the average count by a predetermined conversion factor based on magnification with a Zeiss epifluorescence microscope. Inclusions were counted individually in cultures with <100 inclusions. All technicians who determined inclusion-forming unit counts used the same standardized method [ 5]. For serotyping, chlamydial strains were cultured in 96-well microtiter plates and were serotyped by using subspecies- and serovar-specific monoclonal antibodies. Serovars were additionally grouped as B complex (serovars B, Ba, D, Da, D ? , E, L1, L2, and L2a), intermediate group FG (serovars F and G), and C complex (serovars A, C, H, I, Ia, I ? , J, K, and L3) [ 6–8]. The nonfusing chlamydial phenotype was detected visually by observing inclusion morphology during serotyping [ 5]
